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It partially stimulates the Brst of bone, muscle and cartilage cells directly. The use of relatively low tidal volumes avoids 862 CHAPTER 21 Regulating the Eukaryotic Cell Cycle (a) Untreated extract Mitotic events Addition of sperm nuclei Time (c) RNase-treated extract wild-type cyclin B mRNA Mitotic events (b) RNase-treated extract Addition of sperm nuclei MPF activity Cyclin B concentration Time (d) RNase-treated extract nondegradable Real binary option strategy Toronto (Mississauga) B mRNA Addition of sperm nuclei Addition of sperm nuclei Time Mitotic arrest Time EXPERIMENTAL FIGURE 21-9 Cycling of MPF activity and mitotic events in Xenopus egg extracts depend on synthesis and degradation of cyclin B.

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